GGA is too High

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(OverSeeding)
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== Incorrectly Made == == Incorrectly Made ==
-This is the most common cause of high GGA. Human beings make mistakes, this is certainly a well known fact. The GGA must be prepared precisely. When Standard Methods says that the GGA must be 150 mg/L of glucose and 150 mg/L of glutamic acid, that is what it must be. Even small changes in this contribution can lead to grossly different BOD values. +This is the most common cause of high [[GGA]]. Human beings make mistakes, this is certainly a well known fact. The [[GGA]] must be prepared precisely. When Standard Methods says that the GGA must be 150 mg/L of glucose and 150 mg/L of glutamic acid, that is what it must be. Even small changes in this contribution can lead to grossly different BOD values.
-One way to avoid this issue altogether is to purchase a commercially prepared GGA. Many different companies prepare GGA. Choose one with the following characteristics:+One way to avoid this issue altogether is to purchase a commercially prepared [[GGA[[. Many different companies prepare [[GGA]]. Choose one with the following characteristics:
-* The GGA should be ampouled not bottled+* The [[GGA]] should be ampouled not bottled
-* The GGA should come from a known supplier with a good reputation+* The [[GGA]] should come from a known supplier with a good reputation
-* The GGA should contain ONLY glucose and glutamic acid (no preservatives).+* The [[GGA]] should contain ONLY glucose and glutamic acid (no preservatives).
* Not premeasured. You should be testing your pipetting technique as well as the seed. * Not premeasured. You should be testing your pipetting technique as well as the seed.
-The following GGA is from a respected source. It is at twice the concentration recommended by Standard Methods which results in superior stability, but does require half the recommended amount. For example if you use 6 mL normally you should use 3 mL instead. +The following [[GGA]] is from a respected source. It is at twice the concentration recommended by Standard Methods which results in superior stability, but does require half the recommended amount. For example if you use 6 mL normally you should use 3 mL instead.
-[http://www.hach.com/hc/search.product.details.invoker/PackagingCode=1486510/NewLinkLabel=BOD+Standard+Solutions+%28NIST%29%2C+300+mg%26frasl%3BL%2C+pk%26frasl%3B16+-+10-mL+Voluette%AE+Ampule/PREVIOUS_BREADCRUMB_ID=HC_SEARCH_KEYWORD/SESSIONID|AlUzTVRNM056VXlPVEV4Sm1kMVpYTjBXbGhFU2xFeE1RPT1Ceg==| Hach Glucose and Glutamic Acid (GGA)]+[http://www.hach.com/hc/search.product.details.invoker/PackagingCode=1486510/NewLinkLabel=BOD+Standard+Solutions+%28NIST%29%2C+300+mg%26frasl%3BL%2C+pk%26frasl%3B16+-+10-mL+Voluette%AE+Ampule/PREVIOUS_BREADCRUMB_ID=HC_SEARCH_KEYWORD/SESSIONID|AlUzTVRNM056VXlPVEV4Sm1kMVpYTjBXbGhFU2xFeE1RPT1Ceg==| Hach Glucose and Glutamic Acid ([[GGA]])]

Revision as of 19:24, 1 September 2006

There are a two main reasons that the GGA might be to high. One is that the GGA might be overseeded and the other is that the GGA might be made incorrectly.


OverSeeding

Although this is not the most common reason for high GGA results it is possible that the [[GGA}]] is overseeded. Why is this a problem? The GGA is expected to be 198 +/- 30.5 mg/L but that is not the theoretical value. The theoretical value is actually about 307 mg/L. It is interely possible that a more robust population of seed organisms could easily consume more than the expected 198 mg/L. So what is the solution in this case? Change your seed source to a less robust seed, or perhaps you could use less seed (keep the oxygen depletion of the seed around 0.6-1.0 mg/L in the bottles that are seeded).

Incorrectly Made

This is the most common cause of high GGA. Human beings make mistakes, this is certainly a well known fact. The GGA must be prepared precisely. When Standard Methods says that the GGA must be 150 mg/L of glucose and 150 mg/L of glutamic acid, that is what it must be. Even small changes in this contribution can lead to grossly different BOD values.

One way to avoid this issue altogether is to purchase a commercially prepared [[GGA[[. Many different companies prepare GGA. Choose one with the following characteristics:

  • The GGA should be ampouled not bottled
  • The GGA should come from a known supplier with a good reputation
  • The GGA should contain ONLY glucose and glutamic acid (no preservatives).
  • Not premeasured. You should be testing your pipetting technique as well as the seed.

The following GGA is from a respected source. It is at twice the concentration recommended by Standard Methods which results in superior stability, but does require half the recommended amount. For example if you use 6 mL normally you should use 3 mL instead.

Hach Glucose and Glutamic Acid (GGA)

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