GGA is too Low

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== UnderSeeding == == UnderSeeding ==
-Although this is not the most common reason for high [[GGA]] results it is possible that the [[GGA}]] is underseeded. The real purpose of the [[GGA]] is to set the seed concentration correctly. Many people have mistakenly thought that if the oxygen depletion of the seed is 0.6 to 1.0 mg/L that is all there is to worry about but this is incorrect. The most important function of the [[GGA]] is to tell us when we have enough seed in our seeded samples. Regardless of the oxygen depletion of the seed the seed amount should be adjusted until 198 mg/L of [[GGA]] is obtained on average.+Although this is not the most common reason for low [[GGA]] results it is possible that the [[GGA}]] is underseeded. The real purpose of the [[GGA]] is to set the seed concentration correctly. Many people have mistakenly thought that if the oxygen depletion of the seed is 0.6 to 1.0 mg/L that is all there is to worry about but this is incorrect. The most important function of the [[GGA]] is to tell us when we have enough seed in our seeded samples. Regardless of the oxygen depletion of the seed the seed amount should be adjusted until 198 mg/L of [[GGA]] is obtained on average.
== Incorrectly Made == == Incorrectly Made ==

Revision as of 17:23, 1 November 2006

There are a two main reasons that the GGA might be to low. One is that the GGA might be underseeded and the other is that the GGA might be made incorrectly.


UnderSeeding

Although this is not the most common reason for low GGA results it is possible that the [[GGA}]] is underseeded. The real purpose of the GGA is to set the seed concentration correctly. Many people have mistakenly thought that if the oxygen depletion of the seed is 0.6 to 1.0 mg/L that is all there is to worry about but this is incorrect. The most important function of the GGA is to tell us when we have enough seed in our seeded samples. Regardless of the oxygen depletion of the seed the seed amount should be adjusted until 198 mg/L of GGA is obtained on average.

Incorrectly Made

This is the most common cause of low GGA. The GGA must be prepared precisely. When Standard Methods says that the GGA must be 150 mg/L of glucose and 150 mg/L of glutamic acid, that is what it must be. Even small changes in this contribution can lead to grossly different BOD values.

One way to avoid this issue altogether is to purchase a commercially prepared GGA. Many different companies prepare GGA. Choose one with the following characteristics:

  • The GGA should be ampouled not bottled
  • The GGA should come from a known supplier with a good reputation
  • The GGA should contain ONLY glucose and glutamic acid (no preservatives).
  • Not premeasured. You should be testing your pipetting technique as well as the seed.

The following GGA is from a respected source. It is at twice the concentration recommended by Standard Methods which results in superior stability, but requires half the amount for the same BOD for example 3 mL of Hach GGA is the same as 6 mL of the formulary in Standard Methods. For example if you use 6 mL normally you should use 3 mL instead.

Hach Glucose and Glutamic Acid (GGA)

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