Super Saturation of the Blank
If the blank water is saturated with oxygen prior to being set to 20 degrees C in an incubator, it is highly likely that the oxygen will stay at a higher level than saturation. In order to correct this do the following.
- Store the dilution water at least one day prior to use.
- At the end of the first day agitate (shake) the carboy to cause the water to become saturated.