Super Saturation of the Blank

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If the blank water is saturated with oxygen prior to being set to 20 degrees C in an incubator, it is highly likely that the oxygen will stay at a higher level than saturation. In order to correct this do the following.

  • Store the dilution water at least one day prior to use.
  • At the end of the first day agitate (shake) the carboy to cause the water to become saturated.
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